A Guide to Next Generation Sequencing Basics and Terminologies | Bioinformatics 101

In this video, I delve into the intricacies of a standard workflow for next-generation sequencing (NGS). We'll explore essential terminologies and concepts, including sequencing library preparation, multiplexing, sample pooling, as well as the roles of adaptors and barcodes. Furthermore, I provide an overview of different generations of sequencing technologies, spanning from the traditional Sanger sequencing to the advancements seen in Illumina, Ion Torrent, PacBio, and Oxford Nanopore sequencing technologies. I hope you find this video helpful! Leave your thoughts in the comment section below! ▸ Bridge Amplification: 1. https://youtu.be/fCd6B5HRaZ8?si=rZAZnBkAwXGu1HU9 2. https://youtu.be/CZeN-IgjYCo?si=7DqAbixA84S3vWE2 ▸ Sequencing by Synthesis (Illumina): 1. https://youtu.be/HMyCqWhwB8E?si=Dv8TYEes8b9aULRW 2. https://youtu.be/oIJaA6h2bFM?si=aPoNguaHmVEQrod0 ▸ What is sequencing depth/depth of coverage? https://youtu.be/1jxDv6qVmLQ ▸ Cost to coverage calculator: 1. https://geneglobe.qiagen.com/us/tools/sequencing-coverage-calculator 2. https://support.illumina.com/downloads/sequencing_coverage_calculator.html ▸ What is a fastq file? https://youtu.be/xYEre9DtIqA Chapters: 0:00 Intro 0:51 What is Next Generation Sequencing? 1:29 Evolution of sequencing technologies 4:10 A typical NGS workflow 5:01 What is library preparation? 5:47 What is a Flow cell? 7:28 What is multiplexing? 8:59 Index vs barcode 9:17 How many samples to multiplex? 10:39 What is a sequencing library? 11:01 Sequencing run 11:35 Output from sequencing run - fastq You can show your support and encouragement by buying me a coffee: https://www.buymeacoffee.com/bioinformagic To get in touch: Website: https://bioinformagician.org/ Github: https://github.com/kpatel427 Email: [email protected] #bioinformagician #bioinformatics #illumina #bridgeamplification #sequencingbysynthesis #multiplex #alleles #10x #oxfordnanopore #pacbio #affymetrix #barcode #setseed #reproducibility #pseudorandom #singleR #singlecell #annotationdbi #reversestranded #directstranded #strandedness #survival #survminer #survivalanalysis #kaplanmeier #tcga #gdcportal #tcgaportal #nci #cran #bioconductor #funcotator #variantcalling #variants #gatk #vcf #gvcf #haplotype #alleles #geneticvariants #mutations #gff3 #gff #gtf #sam #bam #phred #fasta #fastq #singlecell #10X #ensembl #biomart #annotationdbi #annotables #affymetrix #microarray #affy #ncbi #genomics #beginners #tutorial #howto #omics #research #biology #GEO #rnaseq #ngs