Bio-layer interferometry is a label-free technology used to find the biomolecular interactions in real-time between two molecules of interest. This technique has significant applications in identifying properties of reaction kinetics, such as rate of association and rate of dissociation. Additionally, due to its ability to achieve real-time high-resolution data, BLI is useful in pharmaceutical research to determine the characteristics of a given drug and its binding to different targets. This video describes the principles and procedure for running a biolayer-interferometry assay.
And do not hesitate to ask questions in the comments! ✍🏻
Molly Shoichet Lab
University of Toronto, Ontario, Canada