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Differential expression analysis

Chipster Tutorials 65,456 8 years ago
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This tutorial covers normalization, dispersion estimation, statistical testing, filtering and multiple testing correction. The materials are available at https://chipster.csc.fi/manual/courses.html#rna We have added some clarifications on (log) fold change and other statistical testing related terms here: https://chipster.rahtiapp.fi/manual/statistical-terms-explained.html 04:02 Normalization 08:02 FPKM method and why you should use raw values 12:20 Dispersion estimation 17:33 Statistical testing (generalized linear models, edgeR, DESeq2) 21:30 Multiple testing correction 23:20 Filtering 24:43 DESeq2 result table

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