Check out our video on how to prepare whole oats for mushroom cultivation
https://youtu.be/gOAeA6eVyR0
Materials:
Laminar Flow Hood/ Still air box (S.A.B). If without these, work in a cleanly environment and turn off all air flow.
Sterilized grain spawn Bag/🫙Jar
🧴70% Isopropyl Alcohol spray bottle or wipes to sanitize.
🧤Gloves/😷 face mask
💉Liquid culture syringe/ 🧫colonized agar
✂️ Scissors (if w/o injection port)
♨️Impulse sealer(if w/o injection port)
✒️Scalpel (if using agar)
🔥Butane torch (is using agar)
Process Overview
1. Sanitize/ wipe down your work space, bag/jar/agar plate.
2. Prepare the 💉 syringe. Flame sterilize the needle by holding it over a flame until it glows red, then let it cool for a few seconds.
3. If no injection port, cut the bag open or carefully open the jar.
With injection port, insert syringe in injection port carefully.
For all, carefully insert 1-2 CC's of liquid culture. (For agar skip)
4. )If without an injection port), close your lid or seal your bag with an Impulse sealer. (For agar skip)
5. (If using agar) , flame sterilize the scalpel by holding it over a flame until it glows red, then let it cool for a few seconds.
6.(For agar) Cut a piece of Agar or 2 the size of a quarter and quickly yet carefully transfer to jar. Close jar and shake
7. Incubate: Store the jar or bag in a warm, dark area for colonization. Aim for 70-75°F to encourage growth without promoting contamination.
8. Monitoring Colonization: Check for growth after a few days. Look for healthy, white mycelium spreading through the grains.
9.(optional) at about 30% colonized do a break and shake. This will speed up the growth of the mycelium.
🛍 Products
Laminar Flow hood(Ebay)
https://www.ebay.com/itm/373964427364
♨️Impulse sealer(Amazon)
https://www.amazon.com/Metronic-Impulse-Sealer-Sealing-Machine/dp/B06XC76JVZ/ref=mp_s_a_1_3_pp?crid=AWLE8Y1ZN3RX&dib=eyJ2IjoiMSJ9.8xNpWWpDZBORxSGb_wLpLzz1nfmHut_AD30BARioQ6v5jFycdzI4sEJMmiYTEFR0FTuZvrlEIOw77_2QT2GgwKnUsVQfH8h9nMWas5nQkLCb0VupLzVsZHs0FTWSUYy6kWlphiA6SH1vyAiCpGp9ClWj1LLFKu4O_IiN7tKQ-ODIjxS4dzqHyR1IzO-3YDkqjFWdoX_VHdxPBUb38kWEQQ.8xIr_BzWdvMpvCahKONQdnJoXUgckgWFAheU5B05gQE&dib_tag=se&keywords=impulse+sealer&qid=1730753168&sprefix=impu%2Caps%2C177&sr=8-3
🔥Butane torch(Amazon)
https://www.amazon.com/Sondiko-Culinary-Refillable-Adjustable-Desserts/dp/B07L8YPYQK/ref=mp_s_a_1_1_sspa?crid=YFY2XZVZ7EB0&dib=eyJ2IjoiMSJ9.KFdUozfPnqR0k-axGt_H-NWafWL3DayvqOvphrej5lDHpMTVbJg1gl6J4BdoyPfoSh6RT9eVK7ZrrREkmP8i0LWPxz71LWK4Y0Lh7RsOB_iCYcZgscrBCuYVKiZrBUYgc-GqDREuej3e1T4eQyxWT7Xjq9sx7Qzw3_Z-8QHZCO3ljHLg5k0fHJh63J-VvYjJNZWrtRoTl4IMuj5TyLhJuA.bufkgKb26ilzwQ3PQGWHo1Ys5-uO2yr5JWfn1fI-0WY&dib_tag=se&keywords=butane+torch&qid=1730753254&sprefix=butane+%2Caps%2C183&sr=8-1-spons&sp_csd=d2lkZ2V0TmFtZT1zcF9waG9uZV9zZWFyY2hfYXRm&psc=1
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