In this video I show you how to make agar culture slants by allowing the agar to cool with the tube laying at an angle, resulting in a large surface area for spreading a culture.
Agar slants are better suited to maintain stock cultures due to the capability of the slant containers to be capped or sealed, preventing the slant from drying out while also preventing contaminants from entering the media. Slant cultures are also manageable to store due to their small size.
Some advantages over agar plates include less storage space used, less cell division, less drying out, and less chance of fruiting in storage.
Agar Recipe:
10 grams of LME + 10 grams of Agar Agar powder + 500 ml boiling water.
*Make sure to mix while hot and dissolve completely before pouring into the tubes. Make sure to work fast so that the agar does not solidify.
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Chapters:
0:00 Culture Slant Intro
0:24 Supplies
0:53 Popsicle Sticks
1:00 Jar Lids
1:12 Making Agar
6:12 Sterilization
7:23 Making The Slant
10:11 Verify Clean Slants
10:52 Inoculation
14:55 Colonization
15:10 Outro
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Music by:
Chillpeach - Mushroom's Life: https://youtu.be/FScEvRbobsE