Molecular Cloning explained for Beginners

This video is a must watch for beginners to understand how molecular cloning works. All steps of a molecular cloning assay are explained here!


References:

Helpful blog article: https://blog.addgene.org/plasmids-101-restriction-cloning?_ga=2.234147166.1542264151.1688288836-946441933.1688288836&_gl=1*1rqjsgs*_ga*OTQ2NDQxOTMzLjE2ODgyODg4MzY.*_ga_5SC3L963MZ*MTY4ODI5Mzk0Ni4yLjEuMTY4ODI5NDAwNS4wLjAuMA


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Music:
Aakash Gandhi – Liquid Time (YouTube Audio Library)
Listen: https://www.youtube.com/watch?v=XnwmwnJoSjc



0:00 Intro
0:39 Vector generation
1:59 Insert generation
2:37 Isolation of vector and insert
3:48 Assembly
4:29 Transformation
4:51 Selection and screening
5:26 Verification

#molecularbiology #cloning #genecloning