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My Favorite Recipe for Making Agar Plates Full Tutorial

Spore n' Sprout 19,192 1 year ago
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Agar plates are very helpful in mushroom cultivation because you can test cultures for contamination by inoculating them with one drop of a spore syringe/LC syringe before attempting to use the syringe on a grow bag. If the agar plate shows clean mycelium after incubating you are good to go but if it shows other contamination growth it is best not to use the syringe. You can use certain techniques to clean up contaminated mushroom cultures while using agar which makes them necessary. They are also great for propagating cultures and storing them. I leave my agar plates on a shelf in a cold room, they are good for 6-12+ months before the thin agar dries up. For longer storage you should use agar slants: https://youtu.be/MSL9sYez-CM?si=tqwcNmXZyxU67uEN In the past I have used other ingredients and different brands of agar and that actually caused contamination on the plates without even inoculating them. I must have wasted 100s of agar plates before figuring out it was the ingredients that was causing the problems. As soon as I started using this recipe in this video, every plate is perfect every time. Mushroom liquid culture and supplies: https://sporensprout.etsy.com 🍄Check out my social links! https://linktr.ee/sporensprout Recommended supplies: Nutrient agar powder 100 grams: https://amzn.to/4bBk44E Nutrient agar powder 500 grams: https://amzn.to/4bBMITA Media bottles: https://amzn.to/49zS3cd Presto pressure cooker: https://amzn.to/3HW3v5T Digital scale: https://amzn.to/48lwbjO Parafilm: https://amzn.to/3HTO3aw 20 pack sterile Petri dishes: https://amzn.to/3UC7agR Premade agar plates: https://amzn.to/48dqB2w *Clicking my affiliate links and making a purchase may result in me earning a small commission, thank you for supporting the channel! If you don’t have a laminar flow hood I recommend making a still air box: https://youtu.be/svUq2OeQNC4?si=DArhtDzRS8Goecsq Re-usable agar plate tutorial: https://youtu.be/qiAUkAGvxJ8?si=U-4Xzm4RfsmRkTvR How to use the agar plates to clone mushrooms: https://youtu.be/EARNzNHAzf0?si=UUDpYCMOHr_9pjMN Join the new mushroom cultivation discord server! https://discord.gg/xY4nFpr3hB Bulk sterile Petri dishes: https://www.carolina.com/lab-dishes/petri-dishes-polystyrene-disposable-sterile-60-x-15-mm-pk-20/741246.pr?question=500+pack+petri+doshes the new exclusive YouTube member channel to get access to perks: https://www.youtube.com/channel/UCzz88U-ru1EuoUztBN0GP0A/join #growyourown #mushrooms

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