Next Generation Sequencing - A Step-By-Step Guide to DNA Sequencing.

Next Generation Sequencing (NGS) is used to sequence both DNA and RNA. Billions of DNA strands get sequenced simultaneously using NGS. Whereas with Sanger Sequencing, only one strand is sequenced at a time. While the Human Genome Project took over 30 years to sequence the human genome for the first time. Now with Next Generation Sequencing, a whole human genome can be sequenced in just one day. This video describes the library preparation process, cluster generation, the sequencing reaction and filtering and alignment of the sequencing data. This video focuses on the primary sequencing method used by Illumina, Sequencing by Synthesis (SBS). ? https://www.clevalab.com ? This video is also a blog post with images, visit: https://www.clevalab.com/post/a-step-by-step-guide-to-ngs ? You may also like: What is PCR and qPCR? - https://youtu.be/rpLSvEbOmqc Visit the ClevaLab Channel Page for more videos: https://www.youtube.com/@ClevaLab ✉️ Sign up to the email list to be notified of new videos: https://www.clevalab.com/email-subscription SOCIAL MEDIA LINKS Twitter: https://twitter.com/_ClevaLab Instagram: https://www.instagram.com/_clevalab Pinterest: https://www.pinterest.com.au/ClevaLab Tiktok: https://www.tiktok.com/@clevalab Facebook: https://www.facebook.com/ClevaLab CHAPTERS: 00:00 From the Human Genome Project to NGS 00:39 NGS vs Sanger Sequencing 01:01 The Basic Principle of NGS 01:23 DNA and RNA Purification and QC 01:38 Library Preparation - The First Step of NGS 02:32 Sequencing by Synthesis and The Sequencing Reaction 03:19 Cluster Generation From the Library Fragment 03:59 Sequencing of the Forward Strand 04:42 The First Index is Read 04:55 The Second Index is Read 05:15 Sequencing of the Reverse Strand 05:25 Filtering and Mapping of the Reads 05:47 Demultiplexing and Mapping to the Reference 06:19 What is Read Depth in NGS? 06:49 How is NGS being used? 07:01 What Types of NGS Applications Are There? #ngs #sequencing #nextgenerationsequencing #ClevaLab