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This video covers the following common PCR issues you may be experiencing, how they might appear on an agarose gel after gel electrophoresis, and solutions to the problems presented.
Unexpected Bands/Primer Dimers- 0:28
Unexpected Bands/Non-specific Binding of Primers- 2:52
Missing Bands on gel- 3:52
No Bands on gel- 4:52
Weak/faint Bands- 6:17
Smeared Bands- 7:03
Products Mentioned
DMSO PCR additive to prevent secondary structures:
https://www.goldbio.com/product/3805/sterile-filtered-dmso
BSA PCR additive to prevent components from sticking to the vial:
https://www.goldbio.com/product/3662/bovine-serum-albumin-bsa-fraction-v-protease-free
Goldbio DNA Ladders:
https://www.goldbio.com/search?q=DNA+ladder
Goldbio Agarose:
https://www.goldbio.com/search?q=Agarose
Goldbio PCR products:
https://www.goldbio.com/collection/pcr-products
Related articles/resources:
PCR Troubleshooting articles 1-3
• Nonspecific binding: https://www.goldbio.com/articles/article/troubleshooting-pcr-part-1-4-pcr-tips-when-encountering-nonspecific-binding
• Not getting any bands: https://www.goldbio.com/articles/article/troubleshooting-pcr-part-two-what-to-do-when-you-are-not-getting-bands
• Weak or smeared bands: https://www.goldbio.com/articles/article/troubleshooting-pcr-part-three-solutions-for-weak-bands-and-smearing
PCR mastermix calculator- https://www.goldbio.com/PCR%20Master%20Mix%20Calculator
25 Lab Hacks You Need to Know: https://www.goldbio.com/articles/article/25-real-lab-hacks-from-researchers-like-you
Interesting PCR application:
PCR Reunites Humans to Ancestors: https://www.goldbio.com/articles/article/ancient-family-reunion-pcr-amplification-reunites-humans-to-ancestors
Gold Biotechnology, Inc.
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